Protoplasting of rice roots

A step-by-step protocol on how to protoplast rice roots for single-cell transcriptomic study and transient gene expression assay.

Author: Mingyuan Zhu, Duke University
Not Published. Please check the link to get access to the detailed protocol.


Isolation and Regeneration of Protoplasts of the Moss

A protocol on how to isolate individual protoplasts from young gametophyte tissue of the moss Physcomitrella patens and how to regenerate them into plants.

Authors: David J. Cove et al. Published on CSH Protocols.
Available on DOI: doi:10.1101/pdb.prot5140

Protoplasting of grass green tissue

A step-by-step protocol on how to protoplast grass green tissue for transformation and omics assay.

Author: Jianqiang Shen, UC Berkeley
Please check the link to get access to the detailed protocol.
Also refer to the relevant publication on Nature communication, 
DOI: https://doi.org/10.1038/ncomms14651